All groups and messages .... Phyloseq offers several ordination methods and distance metrics. Includes control for genetic drift for bi. ... adonis is a function for the analysis and partitioning sums of squares using semimetric and metric distance matrices. 000 - high 0., 2001) was conducted to evaluate the effect among a population by using the pairwise Adonis package. Sequence tables were finally analysed using Phyloseq version 1.24.0 (McMurdie and Holmes, 2013). Samples were not subsampled as dada2 is already removing spurious reads from the data and as subsampling is known to introduce high rates of false positives (McMurdie and Holmes, 2014). However, Miseq-generated samples with less than 1700 reads were.